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Alternatively, DNA hybridization can be arranged in a " zipper " geometry, where single nucleotide pairs are broken sequentially, thus requiring lower forces (Albrecht et al. 2003; Kufer et al. 2008).

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Naked DNA—a nonreplicating, nonintegrating polynucleotide which codes for a vaccine antigen, therapeutic protein, or immunotherapeutic protein, which may be operatively linked to a promoter and inserted into a replication competent plasmid. The DNA is free from association with transfection facilitating proteins, viral particles, liposomal ...